Skip to main content

Table 2 Co-amplification results of RVFV RNA and S. aureus DNA.

From: Phi29 polymerase based random amplification of viral RNA as an alternative to random RT-PCR

  

Staphylococcus aureus

 

Amount input Nucleic Acid

1.3 × 104 copies

1.3 × 106 copies

  

Final yield in copies

Rift Valley Fever Virus

1.5 × 10 4 copies

SA: 2.1 ± 0.9 × 109

RVFV: 2.1 ± 0.3 × 1010

SA: 1.4 ± 0.1 × 1011

RVFV: 3.8 ± 2.7 × 107

 

1.5 × 10 6 copies

SA: 8.2 ± 1.7 × 108

RVFV: 1.37 ± 0.03 × 1011

SA: 1.4 ± 0.2 × 1011

RVFV: 2.5 ± 0.7 × 109

  1. All the quantifications, both before and after amplification, were determined by qPCR as described in M&M.
  2. SA: S.aureus