Table 1 Call rates for seven different viral RNAs using WTA method.
From: Phi29 polymerase based random amplification of viral RNA as an alternative to random RT-PCR
Whole Transcriptome Amplification | |||||
|---|---|---|---|---|---|
Viral family | Name of virus | Number of fragments (size) | Strand | Call rate (%) | |
Average ± SD | Range (Min-Max) | ||||
Coronaviridae | SARS-CoV1 | 1 (29751) | S + | 99.1 ± 0.3 | (98.9–99.3) |
Flaviviridae | Yellow fever | 1 (10862) | S + | 74.4 ± 2.5 | (72.6–76.2) |
Dengue type 2 | 1 (10703) | S + | 59.4 ± 10.7 | (51.8–66.9) | |
Rhabdoviridae | VSV2 | 1 (11161) | S - | 96.8 ± 2.1 | (95.3–98.3) |
CVS3 | 1 (11966) | S - | 73.6 ± 1.1 | (72.8–74.3) | |
Bunyaviridae | Rift valley fever virus | 3 (12181: 6606*, 3885, 1690) | S +/- | 100 ± 0 | (100–100) |
Orthomyxoviridae | Influenza virus type B (B/Yamagata/166/98) | 8 (14289: 2328*, 2352, 2273, 1843,1793, 1504, 1151, 1045) | S - | 72.3 ± 9.7 | (65.4–79.1) |