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Table 4 Details of candidate endogenous control (EC) genes and their PCR amplification efficiencies

From: Identification of suitable endogenous control genes for microRNA gene expression analysis in human breast cancer

Name

Mature

length

(nt)

RNA

species

Accession

number

Function

Reference

PCR

Amplification

efficiency (%)

let-7a

22

miRNA

MI0000060*

Negatively regulates RAS oncogene

[57]

96.3

miR-10b

22

miRNA

MI0000267 *

No functionally-verified targets

 

104.1

miR-16

22

miRNA

MI0000070 *

Negatively regulates B-cell lymphoma mRNA in chronic lymphocytic leukaemia patients

[7]

104.3

miR-21

22

miRNA

MI0000077 *

Antiapoptotic, negatively regulates apoptosis-related genes

[42]

96.8

miR-26b

22

miRNA

MI0000084 *

No functionally-verified targets

 

98.1

RNU19

198

snoRNA

X94290 **

May be involved in pre-rRNA processing

[59–61]

99.2

RNU48

63

snoRNA

NR_002745 **

Guides the 2'O-ribose methylation of 28S rRNA

[62]

108.9

Z30

97

snoRNA

AJ007733 **

Guides the methylation of the Am47 residue in U6 snRNA

[63]

104.1

  1. *mirBase database accession number ** Entrez gene ID