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Table 4 Details of candidate endogenous control (EC) genes and their PCR amplification efficiencies

From: Identification of suitable endogenous control genes for microRNA gene expression analysis in human breast cancer

Name Mature
length
(nt)
RNA
species
Accession
number
Function Reference PCR
Amplification
efficiency (%)
let-7a 22 miRNA MI0000060* Negatively regulates RAS oncogene [57] 96.3
miR-10b 22 miRNA MI0000267 * No functionally-verified targets   104.1
miR-16 22 miRNA MI0000070 * Negatively regulates B-cell lymphoma mRNA in chronic lymphocytic leukaemia patients [7] 104.3
miR-21 22 miRNA MI0000077 * Antiapoptotic, negatively regulates apoptosis-related genes [42] 96.8
miR-26b 22 miRNA MI0000084 * No functionally-verified targets   98.1
RNU19 198 snoRNA X94290 ** May be involved in pre-rRNA processing [5961] 99.2
RNU48 63 snoRNA NR_002745 ** Guides the 2'O-ribose methylation of 28S rRNA [62] 108.9
Z30 97 snoRNA AJ007733 ** Guides the methylation of the Am47 residue in U6 snRNA [63] 104.1
  1. *mirBase database accession number ** Entrez gene ID
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