Skip to main content

Table 2 Ranking of candidate EC gene and choice of best pair of EC genes by NormFinder and geNorm programmes

From: Identification of suitable endogenous control genes for microRNA gene expression analysis in human breast cancer

Rank

NormFindera

geNormb

 

Gene

Stability

Gene

Stability (M)

1

let-7a

0.312

let-7a

1.427

2

miR-16

0.379

miR-16

1.473

3

RNU48

0.401

miR-26b

1.538

4

miR-26b

0.425

RNU48

1.567

5

miR-10b

0.435

miR-10b

1.667

6

miR-21

0.601

miR-21

1.692

7

Z30

0.624

Z30

2.272

Best combination

let-7a/RNU48

0.221

let-7a/miR-16

0.978

  1. Greater expression stability is indicated by a lower stability value (M). Results for seven EC candidates are given as RNU19 was excluded from analysis due to its low expression in the breast tissues. For NormFinder analysis, breast tissue samples were grouped into metastases-free (MF), bone metastases (BM), visceral and bone metastases (VBM) and benign (BEN). The stability is calculated from the intra- and inter-group variation and the best combination of EC genes is also given. b GeNorm stability is based on an estimate of the pairwise variation (M).