Results of Agilent Bioanalyser Small RNA assay. (A) Virtual gel-numbered samples refer to malignant breast tissues (as per Table 2), L = Ladder, N = Normal breast tissue, BEN = benign breast tissue. The lower marker is visible at 4 nt. Samples with a large percentage (> 75%) of miRNA (10–35 nt) include the samples BEN1, 19 and 25, the latter two belonging to the bone metastases (BM) patient group. The high intensity band in samples 9, 15, 17, 20, BEN1 and BEN2 between 60 and 80 nt represents high recovery of tRNA (73–93 nt). In general, these samples had a lower percentage of miRNA. (B) Electropherogram. Numbered samples refer to malignant breast tissues (as per Table 2), BEN = benign breast tissue.