Table 2 Candidate housekeeping genes tested in this study.
From: Normalisation genes for expression analyses in the brown alga model Ectocarpus siliculosus
Gene symbol | Homologous to | Description of trace archive | Accession number | Oligonucleotides – Forward – Reverse | E (%) | R2 | Tm product | PCR product length (bp) |
|---|---|---|---|---|---|---|---|---|
ACT | Actin | KY0AIB94YO18AHM1 | 1927036313 | CCCAGATCATGTTCGAGACGTT CACGCCGTCACCCGAGTC | 91 | 1.000 | 87.80 | 119 |
ARP2.1 | p34-arc subunit of the actin-related protein complex ARP2/3 | KY0AFIPA38YJ23RM1 | 1927195696 | GAAGGAGTTCTGCCGGGAAG ACAAAGCAGCAACGCAGAGA | 98 | 0.994 | 84.50 | 121 |
ARP2.2 | ARP2 subunit of the actin-related protein complex ARP2/3 | KY0AIB269YJ02AHM1 | 1929831232 | GAAGAAGTTCAAGCTCAACATCGA CCGCACCCCCAATGAAA | 104 | 0.998 | 80.90 | 68 |
CYC | Cyclophilin | KY0ADB29YF06FM1 | 1291599781 | AGACGGCGGTGCAAGTAGG GTGAGTCACGGCTGCTTTTATG | 92 | 0.997 | 84.80 | 101 |
Dyn | Dynein light chain protein | KY0AEB344YP09RM1 | 1306215256 | GGAACAAAGCATGGTGACAACA CGCGTGCCTATCCAAGCT | 100 | 0.999 | 81.20 | 65 |
EF1alpha | Translation elongation factor 1 alpha | KY0AEC342YI10RM1 | 1291335619 | GCAAGGGCCTCAGCTCTG ACAAGCCGTCTGGGTATATGTTAGC | 92 | 0.997 | 81.50 | 160 |
G6PD | Glucose 6 phosphate dehydrogenase | KY0AEF243YN02RM1 | 1299896231 | GTGAGGATGTTCAGGTCCCAG GTGGAAGACCCGGTGAGGT | 90 | 0.996 | 84.50 | 101 |
IF2A | Translation initiation factor eIF2 alpha | KY0AIB251YB11AHM1 | 1918199315 | GCGGTACGTGATGGACACC CCCCCGACTCGATGATCTTT | 94 | 0.991 | 84.80 | 101 |
IF4E | Translation intiation factor eIF4E | KY0ADA42YE14FM1 | 1291478318 | TCGCGATTCGAGGTTTGAGTA CAAACGCTGCGGCAGC | 100 | 0.991 | 82.40 | 71 |
R26S | Ribosomal protein 26S | KY0AEC624YL15RM1 | 1300144654 | GCTAGGCTTGCGTTTGTGTG GGCGAGACAGAAAGATTCCG | 93 | 0.995 | 85.40 | 101 |
TUA | Alpha tubulin | KY0AEC614YE14RM1 | 1299935912 | TTTGAGGAGTTTCGTCGGAGAT CACACAGCGCAAAACGGC | 92 | 0.999 | 83 | 140 |
UBCE | Ubiquitin conjugating enzyme | KY0AFIPA87YJ24RM1 | 1917772478 | AACAATGGCCTTTGCGAAAA GCGTACGTCTTGAAGCCCAG | 95 | 0.997 | 84.50 | 101 |
UBQ | Ubiquitin C | KY0AEC576YH18FM1 | 1306241438 | CAACGCCCATGATTGTTCAC GATTATTCCCATCCACGGCA | 100 | 0.997 | 82.70 | 101 |
mN | Intron amplification | KY0AEF302YN21FM1 | 1306150449 | TCATTTTTCATGTGGAGGTCTCTG GCCAAACAAACAACAACCCTC | 83 | 0.981 | 84.80 | 93 |