Figure 2
From: Differential expression of the TFIIIB subunits Brf1 and Brf2 in cancer cells
The Brf1 and Brf2 promoters differ in activity in breast, cervical and prostate cancer cell lines. (A) Schematic representation of the Brf1 promoter identified. The empty pGL3 vector (300 ng), as well as increasing concentrations of Brf1-pGL3 (100 ng, 200 ng, 300 ng) and Brf2-pGL3 (100 ng, 200 ng, 300 ng) were transiently transfected into asynchronous (A) HeLa, (B) DU145, and (C) MCF-7 cells. All luciferase assay results are expressed as relative light units (RLU): the average of the Photinus pyralis firefly activity observed divided by the average of the activity recorded from the Renilla luciferase vector. Experiments were done in triplicate, repeated three times, and representative experiments are depicted.