Figure 1
From: Differential expression of the TFIIIB subunits Brf1 and Brf2 in cancer cells
The TFIIIB subunits Brf1 and Brf2 are differentially expressed in cancer cell lines. Total RNA was isolated from asynchronous growing Caski, HeLa, RKO, SIHA, Du 145, ZR75-1, MCF-7, MDA-MB-453, MDA-MB-231, and MDA-MB-468 cells. After first strand cDNA synthesis, diluted cDNA was used in PCR using the primers depicted in Table 1. The mRNA expression of (A) β-actin, (B) Brf1, and (C) Brf2 are shown. The expected sizes of the PCR products of the different TFIIIB subunits are depicted.