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Table 2 Excision frequencies for donor plasmids

From: Mutational analysis of highly conserved aspartate residues essential to the catalytic core of the piggyBac transposase

 

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REN Screen

Mutant

Excision Frequency

+/- SE

Excision Frequency

+/- SE

D268E

0.00E+00

0.00E+00

9.96E-03

3.73E-03

D268N

0.00E+00

0.00E+00

8.03E-05

4.73E-05

D346E

0.00E+00

0.00E+00

8.97E-05

4.90E-05

D346N

2.97E-05

1.49E-05

3.62E-04

2.02E-04

D447E

4.44E-05

8.54E-06

2.40E-04

1.84E-04

D447N

0.00E+00

0.00E+00

3.99E-04

2.93E-04

D450E

8.80E-04

1.36E-04

0.00E+00

0.00E+00

D450N

1.38E-05

1.38E-05

1.25E-03

0.00E+00

+ctrl

1.40E-02

2.12E-03

3.15E-03

8.86E-04

-ctrl

0.00E+00

0.00E+00

00E+00

0.00E+00

  1. Values obtained for the frequency of excision using the two different donor plasmids with each of the mutant transposases. These numbers are normalized to a positive control frequency of 100%.