Figure 14
Western blot of piggyBac mutant transposases. A western blot was performed on each piggyBac mutant with 125 μg total cell lysate per lane. The top row was probed with anti-piggyBac antibody and indicates the presence of the piggyBac transposase at 68 kDa in each mutant, the positive control, phsp-pBac, and a lack of transposase in the negative control. The bottom row was probed for actin, a 43 kDa protein, using anti-actin I-19 and shows equal loading in all lanes.