Figure 11

PCR analysis of donor plasmid sizes from the blue/white colony screen. PCR of 10 white (negative, top row) colonies and 10 blue (positive, bottom row) colonies, randomly chosen, from the blue/white colony screen is shown on a .9% TAE agarose gel stained with ethidium bromide. The amplicon crosses the entire donor fragment. Expected band size for the pre-excision plasmid is 1027 bp and 202 bp for the post-excision donor plasmid amplicon.