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Table 1 Primers and oligos used in this study

From: Analysis of the piggyBac transposase reveals a functional nuclear targeting signal in the 94 c-terminal residues

 

Primer 1

Primer 2

pMT/EYFP

ACTGGAATTCATGGTGAGCAAGGGCGAGGAGCTG

CTAGAGTCGCGGCCGCTTTACTTGTA

pMT/pBac-EYFP

TAGAATTCTCGTGACTAATATATAATAAAATGGGT

ATTAGTGAATTCGAAACAACTTTGGCACATATC

pMT/NLS-1

AAGAATTCGGGATGGCTTCAAAGTCCACGAGGCGTAGC

ATTAGTGAATTCGAAACAACTTTGGCACATATC

pMT/NLS-2

CAGAATTCGTCATGGATCGATCTTTGTCAATGGTGTA

ATTAGTGAATTCGAAACAACTTTGGCACATATC

pMT/NLS-3

TGGAATTCAACATGCGTACGAAGTATATGATAAATGGA

ATTAGTGAATTCGAAACAACTTTGGCACATATC

pMT/NLS-4

TTGAATTCAACATGGCCCTTACTCTCGTCTCATATAAA

ATTAGTGAATTCGAAACAACTTTGGCACATATC

pMT/NLS-5

AGGAATTCAGTATGGAAAAATTTATGAGAAACCTTTAC

ATTAGTGAATTCGAAACAACTTTGGCACATATC

pMT/NLS-6

TAGAATTCTCGTGACTAATATATAATAAAATGGGT

CGGAATTCAACCTTTTCTCCCTTGCTACTGAC

pMT/NLS-7

TAGAATTCTCGTGACTAATATATAATAAAATGGGT

AGGAATTCGGGTCCGTCAAAACAAAACATCG

pMT/NLS-8

TAGAATTCTCGTGACTAATATATAATAAAATGGGT

GTGAATTCGTCACACATCATGAGGATTTTTAT

pMT/NLS-9

TAGAATTCTCGTGACTAATATATAATAAAATGGGT

AGGAATTCTGTGGACATGTGGTTATCTTTTCT

pMT/NLS-10

TAGAATTCTCGTGACTAATATATAATAAAATGGGT

GTGAATTCTGAAGTTGACCAACAATGTTTATT

pMT/NLS-11

ATATGGATCCGCATCGTGCAAAAAATGCAAAAAAGTT

TTTGGATCCCTCTTCAGTACTGTCATCTGATGTACC

pMT/NLS-13

TTTGGATCCATTTGCCTTTCGCCTTATTTTAGAGGGGC

AAAGGATCCGAAATGGTGAGCAAGGGCGAGGAGCTG

pMT/NLS-14

CCCGGATCCAACCTTTTCTCCCTTGCTACTGACATTATGGC

CCCGGATCCCCAGTAATGAAAAAACGTACTTACTGTACTTACTGCCCC

pMT/NLS-15

TTTTGAGCTCAACCTTTTCTCCCTTGCTACTGACATTATGGC

TTTTGAGCTCCCTACTTTGAAGAGATATTTGCGCGAT

pMT/NLS-16

TTTTGAGCTCAACCTTTTCTCCCTTGCTACTGACATTATGGC

TTTTGAGCTCCCAAATGAAGTGCCTGGTACATCAGATG

pMT/NLS-17

TTTTGAGCTCAACCTTTTCTCCCTTGCTACTGACATTATGGC

TTTTGAGCTCCCTACTTTGAAGGCCTATTTGGCCGATAATATCTCTAATATTTTG

pMT/NLS-18

TTTTGAGCTCAACCTTTTCTCCCTTGCTACTGACATTATGGC

TTTTGAGCTCCCTACTTTGGCCGCTTATTTGGCCGATAATATCTCTAATATTTTG

pMT/NLS-12

AATTCGTAATGGGGCCAGTAATGAAAAAACGTACTTACTGTACTTACTGCCCCTCTAAAATAAGGCGAAAGGCAAATG

 
 

AATTCATTTGCCTTTCGCCTTATTTTAGAGGGGCAGTAAGTACAGTAAGTACGTTTTTTCATTACTGGCGCCATTACG

 
  1. Primers used to make each of the vectors described in the text. Vector names are listed on the left with the cooresponding primers used to make the vector given on the right. In the case of pMT/NLS-12, ordered oligos were used as part of the final vector and not for PCR priming, as described in materials and methods.