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Figure 2 | BMC Molecular Biology

Figure 2

From: Analysis of the piggyBac transposase reveals a functional nuclear targeting signal in the 94 c-terminal residues

Figure 2

Confocal microscopy. Confocal microscope images for vectors described in the text. Vector names and their corresponding images are shown. The first column is a transmitted black and white image of the cell. The second column shows EYFP fluorescence pattern observed as a fusion protein with the piggyBac TPase. The third column is the nuclear stain Draq5 while the fourth column is an overlay of the EYFP fluorescence and Draq5 stain. All microscopy work was performed approximately 48 hours post induction. All images are the result of 6 lines averages performed by the imaging software. Each image is zoomed and cropped on the cell or cells of interest but all remain otherwise unenhanced and unaltered.

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