Figure 5
From: In vivo analysis of Caenorhabditis elegans noncoding RNA promoter motifs
The UM2-snoRNA transcript structure. (A) The H/ACA snoRNA locus CeN55 is located in an intron of the protein coding gene D1046.1. Tiling array probe signal intensities indicate that the UM2 sequence upstream of snoRNA CeN55 might also be expressed (red box). (B) Model of RACE amplification of the putative UM2-snoRNA dicistronic fragments. 3AD is a 3'end adaptor ligated to the ncRNAs; 3RT is the primer complementary to 3AD, primer_UM2 located in the region of UM2. The RACE analysis was performed by reverse transcription using the 3RT as primer followed by PCR with primers primer_UM2 and 3RT. (C) Gel analysis of the RACE fragments.