Figure 6From: Efficient four fragment cloning for the construction of vectors for targeted gene replacement in filamentous fungiPacI/Nt.Bbv.CI digested and GFX purified vectors. The designed vectors digested with PacI/Nt.BbvCI and GFX-purified from solution. Lane 1–6: 2log ladder, pRF-HU, pRF-HU2, pRF-HUE, pRF-HU2E and 2log ladder. Expected sizes of bands: pRF-HU (6336 bp), pRF-HU2 (2489 bp and 3834 bp), pRF-HUE (8709 bp) and pRF-HU2E (3840 bp and 4856 bp).Back to article page