Figure 4From: Efficient four fragment cloning for the construction of vectors for targeted gene replacement in filamentous fungiConstruction of USER compatible vectors. Flow chart for the different components used during construction of the USER compatible vectors. The UCS at the left (LB) and right (RB) borders were ordered as complementary oligomers with additional bases at the ends to make them compatible with the vector ends following digestion with the used restriction enzymes.Back to article page