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Figure 1 | BMC Molecular Biology

Figure 1

From: Efficient four fragment cloning for the construction of vectors for targeted gene replacement in filamentous fungi

Figure 1

Strategies for construction of replacement vectors. On the left, the classical strategy for construction of replacement vectors is shown. It consists of two successive restriction and ligation based cloning steps. On the right the single four fragment USER friendly cloning method is shown. The figures are not drawn to scale. hph = hygromycin phospho-transferase expression cassette (selection marker).

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BMC Molecular Biology

ISSN: 1471-2199

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