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Table 2 GlaI activity on methylated oligonucleotide duplexes

From: Substrate specificity of new methyl-directed DNA endonuclease GlaI

Oligonucleotide duplex

The central part of structure of a duplex

Relative enzyme's activity (%) and standard error

G7*/G8

5'-TTTTCGMGM TGACG-3'

100 ± 5

 

3'-AAAAGMGMG ACTGC-5'

 

G8*/G7

5'-CGTCAGMGM GAAAA-3'

97 ± 5

 

3'-GCAGTMGMG CTTTT-5'

 

G3*/G8

5'-TTTTCGMGC TGACG-3'

28 ± 2

 

3'-AAAAGMGMG ACTGC-5'

 

G8*/G3

5'-CGTCAGMGM GAAAA-3'

40 ± 2

 

3'-GCAGTCGMG CTTTT-5'

 

G4*/G7

5'-CGTCAGMGC GAAAA-3'

31 ± 2

 

3'-GCAGTMGMG CTTTT-5'

 

G7*/G4

5'-TTTTCGMGM TGACG-3'

36 ± 2

 

3'-AAAAGCGMG ACTGC-5'

 

G11*/G12

5'-TTTTCAMGM TGACG-3'

61 ± 3

 

3'-AAAAGTGMG ACTGC-5'

 

G12*/G11

5'-CGTCAGMGT GAAAA-3'

60 ± 3

 

3'-GCAGTMGMA CTTTT-5'

 

G6*/G7

5'-CGTCAGCGM GAAAA-3'

16 ± 1

 

3'-GCAGTMGMG CTTTT-5'

 

G7*/G6

5'-TTTTCGMGM TGACG-3'

17 ± 1

 

3'-AAAAGMGCG ACTGC-5'

 

G5*/G8

5'-TTTTCGCGM TGACG-3'

17 ± 1

 

3'-AAAAGMGMG ACTGC-5'

 

G8*/G5

5'-CGTCAGMGM GAAAA-3'

17 ± 1

 

3'-GCAGTMGCG CTTTT-5'

 

G13*/G14

5'-TTTTCGTGM TGACG-3'

21 ± 1

 

3'-AAAAGMAMG ACTGC-5'

 

G14*/G13

5'-CGTCAGMAM GAAAA-3'

16 ± 1

 

3'-GCAGTMGTG CTTTT-5'

 

G20*/G21

5'-TTTTCGMAM TGACG-3'

17 ± 1

 

3'-AAAAGMGTG ACTGC-5'

 

G21*/G20

5'-CGTCAGTGM GAAAA-3'

25 ± 1

 

3'-GCAGTMAMG CTTTT-5'

 

G3*/G4

5'-TTTTCGMGC TGACG-3'

5 ± 1

 

3'-AAAAGCGMG ACTGC-5'

 

G4*/G3

5'-CGTCAGMGC GAAAA-3'

6 ± 1

 

3'-GCAGTCGMG CTTTT-5'

 

G12*/G17

5'-CGTCAGMGT GAAAA-3'

28 ± 1

 

3'-GCAGTCGMA CTTTT-5'

 

G17*/G12

5'-TTTTCAMGC TGACG-3'

13 ± 1

 

3'-AAAAGTGMG ACTGC-5'

 

G15*/G16

5'-TTTTCAMGT TGACG-3'

26 ± 1

 

3'-AAAAGTGMA ACTGC-5'

 

G16*/G15

5'-CGTCAAMGT GAAAA-3'

23 ± 1

 

3'-GCAGTTGMA CTTTT-5'

 

G18*/G19

5'-TTTTCGMGT TGACG-3'

12 ± 1

 

3'-AAAAGCGMA ACTGC-5'

 

G19*/G18

5'-CGTCAAMGC GAAAA-3'

14 ± 1

 

3'-GCAGTTGMG CTTTT-5'

 

G7*/G2

5'-TTTTCGMGM TGACG-3'

< 1

 

3'-AAAAGCGCG ACTGC-5'

 

G2*/G7

5'-CGTCAGCGC GAAAA-3'

< 1

 

3'-GCAGTMGMG CTTTT-5'

 

G13*/G35

5'-TTTTCGTGM TGACG-3'

< 1

 

3'-AAAAGMACG ACTGC-5'

 

G35*/G13

5'-CGTCAGCAM GAAAA-3'

< 1

 

3'-GCAGTMGTG CTTTT-5'

 

G5*/G6

5'-TTTTCGCGM TGACG-3'

< 1

 

3'-AAAAGMGCG ACTGC-5'

 

G6*/G5

5'-CGTCAGCGM GAAAA-3'

< 1

 

3'-GCAGTMGCG CTTTT-5'

 

G12*/G32

5'-CGTCAGMGT GAAAA-3'

< 1

 

3'-GCAGTMGCA CTTTT-5'

 

G32*/G12

5'-TTTTCACGM TGACG-3'

< 1

 

3'-AAAAGTGMG ACTGC-5'

 

G33*/G34

5'-TTTTCATGM TGACG-3'

< 1

 

3'-AAAAGTAMG ACTGC-5'

 

G34*/G3

5'-CGTCAGMAT GAAAA-3'

< 1

 

3'-GCAGTMGTA CTTTT-5'

 

G13*/36

5'-TTTTCGTGM TGACG-3'

< 1

 

3'-AAAAGCAMG ACTGC-5'

 

G36*/13

5'-CGTCAGMAC GAAAA-3'

< 1

 

3'-GCAGTMGTG CTTTT-5'

 

G3*/G6

5'-TTTTCGMGC TGACG-3'

< 1

 

3'-AAAAGMGCG ACTGC-5'

 

G6*/G3

5'-CGTCAGCGM GAAAA-3'

< 1

 

3'-GCAGTCGMG CTTTT-5'

 

G4*/G5

5'-CGTCAGMGC GAAAA-3'

< 1

 

3'-GCAGTMGCG CTTTT-5'

 

G5*/G4

5'-TTTTCGCGM TGACG-3'

< 1

 

3'-AAAAGCGMG ACTGC-5'

 

G22*/G23

5'-TTTTCMMGM TGACG-3'

< 1

 

3'-AAAAGGGMG ACTGC-5'

 

G23*/G22

5'-CGTCAGMGG GAAAA-3'

< 1

 

3'-GCAGTMGMM CTTTT-5'

 

G24*/G25

5'-TTTTCGGGM TGACG-3'

< 1

 

3'-AAAAGMMMG ACTGC-5'

 

G25*/G24

5'-CGTCAGMMM GAAAA-3'

< 1

 

3'-GCAGTMGGG CTTTT-5'

 

G28*/G29

5'-TTTTCTMGM TGACG-3'

< 1

 

3'-AAAAGAGMG ACTGC-5'

 

G29*/G28

5'-CGTCAGMGA GAAAA-3'

< 1

 

3'-GCAGTMGMT CTTTT-5'

 

G30*/G31

5'-TTTTCGAGM TGACG-3'

< 1

 

3'-AAAAGMTMG ACTGC-5'

 

G31*/G30

5'-CGTCAGMTM GAAAA-3'

< 1

 

3'-GCAGTMGAG CTTTT-5'

 

G37*/G38

5'-TTTTCTMGA TGACG-3'

< 1

 

3'-AAAAGAGMT ACTGC-5'

 

G38*/G37

5'-CGTCATMGA GAAAA-3'

< 1

 

3'-GCAGTAGMT CTTTT-5'

 

G26*/G23

5'-TTTTCCMGM TGACG-3'

< 1

 

3'-AAAAGGGMG ACTGC-5'

 

G23*/G26

5'-CGTCAGMGG GAAAA-3'

< 1

 

3'-GCAGTMGMC CTTTT-5'

 

G27*/G24

5'-CGTCAGMCM GAAAA-3'

< 1

 

3'-GCAGTMGGG CTTTT-5'

 

G24*/G27

5'-TTTTCGGGM TGACG-3'

< 1

 

3'-AAAAGMCMG ACTGC-5'

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