From: Substrate specificity of new methyl-directed DNA endonuclease GlaI
Oligonucleotide duplex | The central part of structure of a duplex | Relative enzyme's activity (%) and standard error |
---|---|---|
G7*/G8 | 5'-TTTTCGMGM TGACG-3' | 100 ± 5 |
 | 3'-AAAAGMGMG ACTGC-5' |  |
G8*/G7 | 5'-CGTCAGMGM GAAAA-3' | 97 ± 5 |
 | 3'-GCAGTMGMG CTTTT-5' |  |
G3*/G8 | 5'-TTTTCGMGC TGACG-3' | 28 ± 2 |
 | 3'-AAAAGMGMG ACTGC-5' |  |
G8*/G3 | 5'-CGTCAGMGM GAAAA-3' | 40 ± 2 |
 | 3'-GCAGTCGMG CTTTT-5' |  |
G4*/G7 | 5'-CGTCAGMGC GAAAA-3' | 31 ± 2 |
 | 3'-GCAGTMGMG CTTTT-5' |  |
G7*/G4 | 5'-TTTTCGMGM TGACG-3' | 36 ± 2 |
 | 3'-AAAAGCGMG ACTGC-5' |  |
G11*/G12 | 5'-TTTTCAMGM TGACG-3' | 61 ± 3 |
 | 3'-AAAAGTGMG ACTGC-5' |  |
G12*/G11 | 5'-CGTCAGMGT GAAAA-3' | 60 ± 3 |
 | 3'-GCAGTMGMA CTTTT-5' |  |
G6*/G7 | 5'-CGTCAGCGM GAAAA-3' | 16 ± 1 |
 | 3'-GCAGTMGMG CTTTT-5' |  |
G7*/G6 | 5'-TTTTCGMGM TGACG-3' | 17 ± 1 |
 | 3'-AAAAGMGCG ACTGC-5' |  |
G5*/G8 | 5'-TTTTCGCGM TGACG-3' | 17 ± 1 |
 | 3'-AAAAGMGMG ACTGC-5' |  |
G8*/G5 | 5'-CGTCAGMGM GAAAA-3' | 17 ± 1 |
 | 3'-GCAGTMGCG CTTTT-5' |  |
G13*/G14 | 5'-TTTTCGTGM TGACG-3' | 21 ± 1 |
 | 3'-AAAAGMAMG ACTGC-5' |  |
G14*/G13 | 5'-CGTCAGMAM GAAAA-3' | 16 ± 1 |
 | 3'-GCAGTMGTG CTTTT-5' |  |
G20*/G21 | 5'-TTTTCGMAM TGACG-3' | 17 ± 1 |
 | 3'-AAAAGMGTG ACTGC-5' |  |
G21*/G20 | 5'-CGTCAGTGM GAAAA-3' | 25 ± 1 |
 | 3'-GCAGTMAMG CTTTT-5' |  |
G3*/G4 | 5'-TTTTCGMGC TGACG-3' | 5 ± 1 |
 | 3'-AAAAGCGMG ACTGC-5' |  |
G4*/G3 | 5'-CGTCAGMGC GAAAA-3' | 6 ± 1 |
 | 3'-GCAGTCGMG CTTTT-5' |  |
G12*/G17 | 5'-CGTCAGMGT GAAAA-3' | 28 ± 1 |
 | 3'-GCAGTCGMA CTTTT-5' |  |
G17*/G12 | 5'-TTTTCAMGC TGACG-3' | 13 ± 1 |
 | 3'-AAAAGTGMG ACTGC-5' |  |
G15*/G16 | 5'-TTTTCAMGT TGACG-3' | 26 ± 1 |
 | 3'-AAAAGTGMA ACTGC-5' |  |
G16*/G15 | 5'-CGTCAAMGT GAAAA-3' | 23 ± 1 |
 | 3'-GCAGTTGMA CTTTT-5' |  |
G18*/G19 | 5'-TTTTCGMGT TGACG-3' | 12 ± 1 |
 | 3'-AAAAGCGMA ACTGC-5' |  |
G19*/G18 | 5'-CGTCAAMGC GAAAA-3' | 14 ± 1 |
 | 3'-GCAGTTGMG CTTTT-5' |  |
G7*/G2 | 5'-TTTTCGMGM TGACG-3' | < 1 |
 | 3'-AAAAGCGCG ACTGC-5' |  |
G2*/G7 | 5'-CGTCAGCGC GAAAA-3' | < 1 |
 | 3'-GCAGTMGMG CTTTT-5' |  |
G13*/G35 | 5'-TTTTCGTGM TGACG-3' | < 1 |
 | 3'-AAAAGMACG ACTGC-5' |  |
G35*/G13 | 5'-CGTCAGCAM GAAAA-3' | < 1 |
 | 3'-GCAGTMGTG CTTTT-5' |  |
G5*/G6 | 5'-TTTTCGCGM TGACG-3' | < 1 |
 | 3'-AAAAGMGCG ACTGC-5' |  |
G6*/G5 | 5'-CGTCAGCGM GAAAA-3' | < 1 |
 | 3'-GCAGTMGCG CTTTT-5' |  |
G12*/G32 | 5'-CGTCAGMGT GAAAA-3' | < 1 |
 | 3'-GCAGTMGCA CTTTT-5' |  |
G32*/G12 | 5'-TTTTCACGM TGACG-3' | < 1 |
 | 3'-AAAAGTGMG ACTGC-5' |  |
G33*/G34 | 5'-TTTTCATGM TGACG-3' | < 1 |
 | 3'-AAAAGTAMG ACTGC-5' |  |
G34*/G3 | 5'-CGTCAGMAT GAAAA-3' | < 1 |
 | 3'-GCAGTMGTA CTTTT-5' |  |
G13*/36 | 5'-TTTTCGTGM TGACG-3' | < 1 |
 | 3'-AAAAGCAMG ACTGC-5' |  |
G36*/13 | 5'-CGTCAGMAC GAAAA-3' | < 1 |
 | 3'-GCAGTMGTG CTTTT-5' |  |
G3*/G6 | 5'-TTTTCGMGC TGACG-3' | < 1 |
 | 3'-AAAAGMGCG ACTGC-5' |  |
G6*/G3 | 5'-CGTCAGCGM GAAAA-3' | < 1 |
 | 3'-GCAGTCGMG CTTTT-5' |  |
G4*/G5 | 5'-CGTCAGMGC GAAAA-3' | < 1 |
 | 3'-GCAGTMGCG CTTTT-5' |  |
G5*/G4 | 5'-TTTTCGCGM TGACG-3' | < 1 |
 | 3'-AAAAGCGMG ACTGC-5' |  |
G22*/G23 | 5'-TTTTCMMGM TGACG-3' | < 1 |
 | 3'-AAAAGGGMG ACTGC-5' |  |
G23*/G22 | 5'-CGTCAGMGG GAAAA-3' | < 1 |
 | 3'-GCAGTMGMM CTTTT-5' |  |
G24*/G25 | 5'-TTTTCGGGM TGACG-3' | < 1 |
 | 3'-AAAAGMMMG ACTGC-5' |  |
G25*/G24 | 5'-CGTCAGMMM GAAAA-3' | < 1 |
 | 3'-GCAGTMGGG CTTTT-5' |  |
G28*/G29 | 5'-TTTTCTMGM TGACG-3' | < 1 |
 | 3'-AAAAGAGMG ACTGC-5' |  |
G29*/G28 | 5'-CGTCAGMGA GAAAA-3' | < 1 |
 | 3'-GCAGTMGMT CTTTT-5' |  |
G30*/G31 | 5'-TTTTCGAGM TGACG-3' | < 1 |
 | 3'-AAAAGMTMG ACTGC-5' |  |
G31*/G30 | 5'-CGTCAGMTM GAAAA-3' | < 1 |
 | 3'-GCAGTMGAG CTTTT-5' |  |
G37*/G38 | 5'-TTTTCTMGA TGACG-3' | < 1 |
 | 3'-AAAAGAGMT ACTGC-5' |  |
G38*/G37 | 5'-CGTCATMGA GAAAA-3' | < 1 |
 | 3'-GCAGTAGMT CTTTT-5' |  |
G26*/G23 | 5'-TTTTCCMGM TGACG-3' | < 1 |
 | 3'-AAAAGGGMG ACTGC-5' |  |
G23*/G26 | 5'-CGTCAGMGG GAAAA-3' | < 1 |
 | 3'-GCAGTMGMC CTTTT-5' |  |
G27*/G24 | 5'-CGTCAGMCM GAAAA-3' | < 1 |
 | 3'-GCAGTMGGG CTTTT-5' |  |
G24*/G27 | 5'-TTTTCGGGM TGACG-3' | < 1 |
 | 3'-AAAAGMCMG ACTGC-5' |  |