Table 2 Characteristics of used primers; LNA probes and amplicon sizes in reverse transcriptase-polymerase chain reaction reaction.
Gene abbreviation | Amplicon size (basepairs) | Sense, antisense primers | LNA probe¥ |
|---|---|---|---|
ACTB | 76 | 5'-attggcaatgagcggttc-3' 5'-ggatgccacaggactccat-3' | #11 |
ARF1 | 70 | 5'-gccactacttccagaacacaca-3' 5'-tcgttcacacgctctctgtc-3' | #56 |
CANX | 108 | 5'-aacaccagaactcaacctgga-3' 5'-tgtcggaagatgaagtgcag-3' | #55 |
G6PD | 75 | 5'-ctggtggccatggagaag-3' 5'-gcatttcaacaccttgacctt-3' | #22 |
GAPDH | 78 | 5'-tccactggcgtcttcacc-3' 5'-ggcagagatgatgaccctttt-3' | #45 |
GNB2L1 | 72 | 5'-gctactaccccgcagttcc-3' 5'-cagtttccacatgatgatggtc-3' | #55 |
GPS1 | 66 | 5'-gcaaccagatccatgtcaagt-3' 5'-tgttggctggagtcagctc-3' | #36 |
PSMB2 | 72 | 5'-agagggcagtggaactcctt-3' 5'-aggttggcagattcaggatg-3' | #50 |
PSMD2 | 68 | 5'-gcctcacccagattgacaag-3' 5'-ggcaagaagagctcctgactta-3' | #82 |
RPL32 | 75 | 5'-gaagttcctggtccacaacg-3' 5'-gcgatctcggcacagtaag-3' | #17 |
INFG | 112 | 5'-ggcattttgaagaattggaaag-3' 5'-tttggatgctctggtcatctt-3' | #21 |
CCL2 | 93 | 5'-agtctctgccgcccttct-3' 5'-gtgactggggcattgattg-3' | #40 |