Figure 2

Strategies for detection DNA methylation using restriction enzymes. Detection of methylation patterns using a single methylation sensitive restriction enzyme. Knowledge of expected restriction sites, from an in silico map, in the absence of methylation, locates methylation sites revealed through actual restriction digestion. A; In silico restriction map; cleavage sites are shown as vertical red bars. B; A hypothetical distribution of DNA methylation sites; green bars. C; Composite restriction map (A + B) incorporating blocking effects from the overlap of restriction with methylation sites; location of missing restriction sites caused by overlap (blue circles).