Table 2 Primer sequences and optimum PCR conditions for amplifying the products
Gene | Forward (5' to 3') Reverse (5' to 3') | Product size (bp) | Primer Used (nM) | Annealing Temp (T°) |
|---|---|---|---|---|
Actb | TCCGCCGCCGGCCCACACT AGTCCTTCTGGCCCATGC | 188 | 0.40 0.40 | 60 |
Gapdh | CAAGTTCCACGGCACGGTCA CTCGGCACCAGCATCACCC | 118 | 0.40 0.40 | 68 |
Hprt1 | ACGTCGAGGACTTGGAAAGGGTGTT GGCCTCCCATCTCCTTCATCACATC | 96 | 0.40 0.40 | 68 |
H2afz | AGAGCCGGCTGCCAGTTCC CAGTCGCGCCCACACGTCC | 85 | 0.20 0.33 | 59 |
Ubc | GTGACACCATCGAGAAT GACACCTCCCCTCAGAC | 173 | 0.40 0.30 | 60 |
Ppia | TCCAGGGTTTATGTGCCAGGGTG CGTTTGCCATGGACAAGATGCC | 137 | 0.30 0.30 | 68 |
Eef1e1 | ACCGCAGAAGAGAAAGCCATAG AGCGATGTAGCCCATAGTAGAGGA | 190 | 0.50 0.50 | 68 |
G6pdx | AGCCCGCCTCCACTGACTCC ACCACGTTGTCCGCCTGCAC | 88 | 0.40 0.30 | 60 |
Tbp | GCTGAATATAATCCCAAGCGGTTTGC AAATCAGCGCTGTGGTTCGTGGCTCTC | 73 | 0.30 0.30 | 68 |
Polr2a | AGACTTCTCGGCCCGCACTG ACTTGGCGCCTGGGTACTGG | 176 | 0.40 0.40 | 68 |
B2m | GCTCCGTCTTGGGCTTG CGGATGAAACCCAGATACATAG | 152 | 0.33 0.33 | 60 |
Pgk1 | TGTTGGTCGGGCGAAGCAG CAGTGTCTCCACCGCCGATG | 149 | 0.50 0.20 | 60 |
Ywhaz | GGTCTGGCCCTTAACTTCTCTGTGTTCTA GCGTGCTGTCTTTGTATGATTCTTCACTT | 142 | 0.50 0.50 | 68 |
POU5F1 | CGAGTGAGAGGCAACTTGG CGGTTACAGAACCACACACG | 125 | 0.45 0.20 | 57 |