Table 2 Oligonucleotides used in the qPCR for the selected Agaricus bisporus genes
From: Statistical modelling of transcript profiles of differentially regulated genes
Gene | Oligonucleotide | Amplicon length (bp) |
|---|---|---|
Cruciform DNA-binding protein | Forward: 5'-CGCTGGTGAAGCTGAGAACA-3' | 74 |
| Â | Reverse: 5'- CAGCGATTTGGTCCGTCATA-3' | Â |
Cytochrome P450II | Forward: 5'-GCCGATATTTTGCTCTGAATGC-3' | 75 |
| Â | Reverse: 5'- GCGCAGGCTTGATATCGAA-3' | Â |
β (1–6) Glucan synthase | Forward: 5'-TCAATCTTCTTGATGCTCATTGC-3' | 70 |
| Â | Reverse: 5'- TGCGCAAACAACCTATTCC-3' | Â |
Glucuronyl hydrolase | Forward: 5'-TGATGGAATTGTACCATGGGATT-3' | 74 |
| Â | Reverse: 5'- AGCGATAGTTGCTGCTGAAGAA-3' | Â |
Riboflavin aldehyde-forming enzyme | Forward: 5'-CGGCAGCGGAGACCATT-3' | 65 |
| Â | Reverse: 5'- TGACTTTCACGTATTTGCTTTGT-3' | Â |
18S rRNA | Forward: 5'-ACAACGAGACCTTAACCTGCTAA-3' | 78 |
| Â | Reverse: 5'- GACGCTGACAGTCCCTCTAAGAA-3' | Â |