Skip to main content

Table 4 Summary and comparison of the top candidate housekeeping genes for each gland state identified by geNorm and NormFinder

From: Selection of housekeeping genes for gene expression studies in the adult rat submandibular gland under normal, inflamed, atrophic and regenerative states

 

geNorm

NormFinder

Unoperated

UBC/ACTB

HPRT

24 hr ligated

YWHAZ/ACTB

HPRT

2 wk ligated

HPRT/ACTB

GAPDH

2 wk ligated + 3 day de-ligated

GAPDH/ACTB

HPRT

  1. For the geNorm program, determination of the optimal number of control genes for normalisation is made. This is calculated through pairwise variation analysis through which Vandesompele et al proposed 0.15 as a cut off value. This optimal number of housekeeping genes is in the order given by the program.