Selection of housekeeping genes for gene expression studies in the adult rat submandibular gland under normal, inflamed, atrophic and regenerative states

Table 3 Housekeeping gene rankings for each tissue state

Housekeeping genes ranked in order of their expression stability
Unoperated	M	24 hr ligated	M	2 wk ligated	M	2 wk ligated + 3 day de-ligated	M
ARBP	0.434	SDHA	0.496	SDHA	0.792	SDHA	0.387
SDHA	0.364	GAPDH	0.417	YWHAZ	0.641	YWHAZ	0.318
YWHAZ	0.328	ARBP	0.352	UBC	0.562	ARBP	0.272
GAPDH	0.243	UBC	0.278	ARBP	0.505	HPRT	0.207
HPRT	0.196	HPRT	0.213	GAPDH	0.359	UBC	0.179
ACTB-UBC	0.155	ACTB-YWHAZ	0.136	ACTB-HPRT	0.226	ACTB-GAPDH	0.124

Increasing stability from top to bottom; the two most stable control genes in tissue state, for example ACTB and UBC in unoperated tissue, cannot be ranked in order because of the required use of gene ratios for gene stability measurements. M is defined as the control gene-stability measure.

ISSN: 1471-2199