Table 2 Characteristics of primer and probe sets
Gene symbol | Context Sequence | Amplicon length | Efficiency (%) | Primer & Probe Set |
|---|---|---|---|---|
ACTB | CTTCCTGGGTATGGAATCCTGTGGC | 91 | 100 | Assay by design (ABI) |
ARBP | TGGCCAATAAGGTGCCAGCTGCTGC | 100 | 90 | Assay by design (ABI) |
GAPDH | CGGGAAACCCATCACCATCTTCCAG | 87 | 102 | Assay by design (ABI) |
HPRT | ACTGGAAAGAACGTCTTGATTGTTG | 100 | 94 | Assay by design (ABI) |
SDHA | CATACTGTTGCAGCACAGGGAGGTA | 59 | 98 | Assay by design (ABI) |
UBC | TGGGTTTGATGGGGAGGTGTCTTAG | 88 | 98 | Assay by design (ABI) |
YWHAZ | GCAACGACGTACTGTCTCTTTTGGA | 104 | 102 | Assay by design (ABI) |