Figure 6

The level of phosphorylated TIF1β/Ser473 is decreased in TPA-induced differentiated K562 cells. K562 cells were treated with 10 ng/ml TPA for 4 days. Phase contrast images of control (left panels, TPA-) and differentiated (right panels, TPA+) K562 cells are shown. Equal amounts of proteins from these cells were collected (Coomassie Blue stain) and TIF1β (20A1) or phosphorylated TIF1β/S473 (S473) were detected by Western hybridization. The relative TIF1β/S473 level of proliferating or TPA-induced differentiated K562 cells (by ImageGauge software, after normalization to individual TIF1β level) is shown. Bars in phase contrast images represent 40 μm (upper panels) and 8 μm (lower panels).