Figure 1From: Off-target effects of siRNA specific for GFPA siRNA directed against GFP specifically targets endogenous genes in human cells. (a) After transfection of the control siRNA directed against GFP, underrepresentation of the off-target genes CYLD and SOAT was detected by expression-microarrays 48 h post-transfection in HEK and HeLa cells. Normalization was performed as described in Additional file 5. (b) Verification of underrepresentation of CYLD and SOAT in HEK and HeLa cells by Real-Time PCR using the same mRNA as template. Expression of genes was normalized to the median of 2 housekeeping genes (PGK, DCTN2).Back to article page