Table 6 Primers and amplicons for each of the 10 HKGs and GmBFT
From: Validation of internal control for gene expression study in soybean by quantitative real-time PCR
Name | Forward Primer Sequence [5'-3'] | Reverse Primer Sequence [5'-3'] | Amplicon Length (bp) | Primer locationa | E(%) | R2 |
|---|---|---|---|---|---|---|
ACT11 | CGGTGGTTCTAT CTTGGCATC | GTCTTTCGCTTCAA TAACCCTA | 142 | D | 98.04 | 0.9979 |
ACT2/7 | CTTCCCTCAGCA CCTTCCAA | GGTCCAGCTTTCA CACTCCAT | 119 | D | 109.56 | 0.9979 |
G6PD | ACTCCTTGATAC CGTTGTCCAT | GTTTGTTATCCGCC TACAGCCT | 126 | D | 98.59 | 0.9990 |
ELF1B | GTTGAAAAGCCA GGGGACA | TCTTACCCCTTGA GCGTGG | 118 | D | 93.15 | 0.9883 |
UBC2 | TCCCCTCACACC CTTCCTC | CCATCCCAAGGGG TGTCAT | 155 | D | 100.60 | 0.9988 |
CYP2 | CGGGACCAGTGTGCTTCTTCA | CCCCTCCACTACAAAGGCTCG | 154 | S | 82.31 | 0.9848 |
ELF1A | GACCTTCTTCGT TTCTCGCA | CGAACCTCTCAAT CACACGC | 195 | D | 98.21 | 0.9981 |
TUB | CCTCGTTCGAAT TCGCTTTTTG | CAACTGTCTTGTC GCTTGGCAT | 161 | S | 100.84 | 0.9956 |
TUA | AGGTCGGAAACT CCTGCTGG | AAGGTGTTGAAGG CGTCGTG | 159 | S | 85.75 | 0.9829 |
UBQ10 | CGCCTCTAATCT CGCAGTTCC | GTTGTCAATGGTG TCGGAGGA | 114 | S | 120.28 | 0.9663 |
GmBFT | CCAAGGGAAATT GTGAGGTA | CTACTAAAAAGCC CCACAGC | 191 | S | 101.65 | 0.9985 |