Figure 9

E2F binding to a candidate E2F binding site in BCL2L11-P1. EMSA on the candidate E2F binding site (CTCTGCGCGCCAGAGG). HEK293 cells were transfected with the E2F expression construct, and nuclear extracts were assayed for capacity for specific binding to the candidate E2F binding site through competition with a specific (E2F) or unspecific (NF-κB) unlabelled competitor double stranded (ds) oligonucleotide. A specific shift is identified (arrow) upon transfection with the E2F expression plasmid, which can only be competed with a specific ds oligonucleotide.