Figure 8
From: Identification of a candidate alternative promoter region of the human Bcl2L11 (Bim) gene
Effects of E2F on BCL2L11-P1 constructs. For simplicity the alias BIM is used in the figure instead of the approved gene symbol BCL2L11. A. Sequence of the -1918 P1 region showing the position of 5' deletion variants. Forward primers used to generate the various P1 5' deletion variants are indicated (arrows). The putative E2F binding site is boxed, and the transcript initiation site is indicated (+1). B. Analysis of the effects of E2F over-expression on pBIM-P1 and 5'deletion variants activity in HEK293 cells. A concentration-dependent inducibility of P1 activity is observed in construct pBIM-P1-1918, -1565 and -1326, which contain a putative E2F responsive element. By contrast, pBIM-P1-1284 and -940 display basal activities comparable to pBCL2L11-P1-1918, but without the capacity for E2F-mediated response, suggesting a loss of E2F responsive element(s). *p < 0.05 and **p < 0.01, Anova: single factor.