Figure 3

A novel putative promoter is identified upstream of known BCL2L11 promoters. A. 5'-RACE on transcribed sequences in BCL2L11-P1 to identify transcript initiation sites in HEK293 cells; the major PCR product, indicated by the asterisk, was sequenced and defined the major transcript initiation site. For or Rev: single primer controls; CTRL refers to the no cDNA control. B. Sequence of the cloned BCL2L11-P1 region; the sequence shaded in grey indicates coverage by 16 independent human ESTs in Genbank. The arrow indicates the transcript initiation site identified in HEK293 cells by 5' RACE. The red box indicates the sequence of the reverse primer employed in the 5'RACE experiments.