Figure 2
From: Identification of a candidate alternative promoter region of the human Bcl2L11 (Bim) gene
A transcript originating from the BCL2L11-P1 region in HEK293 cell is a novel BCL2L11 transcript. A. RT-PCR in HEK293 cDNA using a Forward primer anchored within Group 1, subgroup B ESTs and a Reverse primer anchored downstream of the translation initiation codon of HsBCL2L11; a ca. 1000 bp fragment (arrow) is amplified from HEK293 cells. For or Rev: single primer controls; CTRL refers to the no cDNA control. No amplification was observed when RNA was used as template (data not shown). B. Sequence of the amplified cDNA fragment and its alignment with ESTs from Group 1, subgroup B (BM676697, BP395271) and from Group 2 (DB151955). The first BCL2L11 coding exon is shaded in grey (BCL2L11 coding sequence in dark grey).