Figure 1

Bioinformatics analysis recognizes known HsBCL2L11(BIM) promoters and identifies a putative promoter region upstream of these. A. BLAST analysis of 13 kb of genomic sequence upstream of HsBCL2L11 ATG codon (NT_022135.15|Hs2_22291, bases 576452 to 505224) vs Genbank ESTs identified transcribed regions upstream of BCL2L11 locus. Three EST groups can be identified. Of these, groups 2 and 3 correspond to transcripts associated with published BCL2L11 promoters (2: human P2; ref. 13; 3: rat P3, ref. 9). Group 1 is not associated with any published vertebrate BCL2L11 promoter sequences. B. Close-up of a ca. 5000 bp region comprising Group 1, 2 and 3 ESTs. The location of the BCL2L11 ATG codon is indicated. One Group 2 EST (DB151955), comprising the first BCL2L11 coding exon, overlaps with Group 1, subgroup B ESTs. Arrowheads indicate the position of RT-PCR primers used to confirm the co-linearity of Group 1, subgroup B ESTs with the first BCL2L11 coding exon.