Skip to main content

Table 1 Expression level of a copia LTR-CAT construct in nine stably transformed lines of Drosophila melanogaster made hemi- or homozygous for the construct

From: LTR retrotransposons and the evolution of dosage compensation in Drosophila

Transformant

Insert Chrom-osomal Location

-------------

CAT ACTIVITY

------------

---------------

RATIO (homo/hemi)

  

Female homozygous

Female hemizygous

Male homozygous

Male hemizygous

 

8-1

2L 40E

lethal

3.03 (0.33)

  

NA

"

    

lethal

2.9 (0.37)

9-1

2L 39A

lethal

0.32 (0.04)

   

"

    

lethal

0.36 (0.06)

27-1

2R 46E

lethal

0.12 (0.03)

   

"

    

lethal

0.11 (0.01)

9-3

3L 80A

3.82 (0.39)

1.50 (0.28)

  

2.55

"

   

4.01 (0.29)

0.98 (0.21)

4.09

9-4

2R 57B

2.27 (0.27)

0.22 (0.03)

  

10.31

"

   

2.42 (0.22)

0.21 (0.05)

11.52

9-6

3L 75C

3.60 (0.23)

0.56 (0.13)

  

6.43

"

   

3.65 (0.05)

0.32 (0.02)

11.41

14-2

4 102B

1.10 (0.35)

0.28 (0.03)

  

3.93

    

1.26 (0.09)

0.26 (0.02)

4.85

14-1

× 16B

3.91 (0.10)

0.91 (0.09)

  

4.3

    

NA

3.29 (0.04)

NA

39-2

× 5A

1.88 (0.13)

0.20 (0.04)

  

9.4

    

NA

1.91 (0.24)

NA

      

Av: 6.88

  1. The results demonstrate a nearly seven-fold average increase in CAT activity in flies homozygous vs. hemizygous for the copia LTR-CAT construct