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Table 5 Comparison of the amplified length and sensitivity of TaqMan, UT and AUDP PCR

From: A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids

Amplicon Length

TaqMan PCR

UT-PCR

AUDP PCR

 

Amplification (Y/N)

Sensitivity (copies)

Amplification (Y/N)

Sensitivity (copies)

Amplification (Y/N)

Sensitivity (copies)

~200 bp

Y

10

Y

10

Y

10

~500 bp

Y

20

Y

20

Y

10

~1000 bp

N

--

N

--

Y

20

~1600 bp

N

--

N

--

Y

50