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Table 4 GM contents quantification of GM samples using AUDP PCR assays

From: A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids

Target gene/event

Sample

Endogenous/exogenous

Ct

Mean copies

SD

GM content (%)

Bias

   

Ct1

Ct2

Ct3

    

Screen specific detection

CaMV35s

5%

exogenous

34.42

34.17

34.08

34.22

0.18

4.33

13.37

  

endogenous

26.9

26.89

26.78

26.86

0.07

  
 

3%

exogenous

35.01

34.84

34.67

34.84

0.17

2.81

6.22

  

endogenous

26.78

26.82

26.93

26.84

0.08

  
 

1%

exogenous

36.56

36.21

36.89

36.55

0.34

0.86

13.87

  

endogenous

26.81

26.79

26.88

26.83

0.05

  

NOS

5%

exogenous

32.46

32.38

32.1

32.31

0.19

4.80

3.92

  

endogenous

26.91

26.73

26.83

26.82

0.09

  
 

3%

exogenous

33.21

33.32

33.07

33.2

0.13

2.67

10.92

  

endogenous

26.81

26.92

26.97

26.9

0.08

  
 

1%

exogenous

34.37

34.29

34.78

34.48

0.26

1.15

15.12

  

endogenous

26.93

26.99

27.13

27.02

0.10

  

Gene-specific detection

Cp4EPSPS

5%

exogenous

31.1

31.32

31.24

31.22

0.11

4.78

4.34

  

endogenous

26.91

26.97

27.06

26.98

0.08

  
 

3%

exogenous

31.89

31.93

32.14

31.99

0.13

2.63

12.35

  

endogenous

26.81

26.93

26.95

26.90

0.08

  
 

1%

exogenous

33.21

33.47

33.11

33.26

0.19

1.18

17.86

  

endogenous

26.93

26.99

27.14

27.02

0.11

  

Cry1A(b)

5%

exogenous

29.32

29.13

28.92

29.123

0.20

4.04

19.22

  

endogenous

25.78

25.81

25.73

25.7

0.04

  
 

3%

exogenous

29.42

29.28

29.35

29.35

0.07

3.50

16.70

  

endogenous

25.69

25.81

25.92

25.81

0.12

  
 

1%

exogenous

31.45

31.39

31.26

31.37

0.10

0.83

16.97

  

endogenous

25.83

25.86

25.91

25.87

0.04

  

Event-specific detection

MON863

5%

exogenous

29.46

29.78

29.39

29.54

0.21

5.56

11.21

  

endogenous

25.84

25.89

25.93

25.89

0.05

  
 

3%

exogenous

30.68

30.37

30.41

30.49

0.17

3.11

3.53

  

endogenous

25.93

25.97

25.99

25.96

0.03

  
 

1%

exogenous

32.13

32.25

32.51

32.27

0.19

1.21

21.13

  

endogenous

26.01

26.13

26.94

26.36

0.51

  

GA21

5%

exogenous

29.81

29.78

29.53

29.71

0.15

5.62

12.37

  

endogenous

26.07

26.09

25.95

26.04

0.08

  
 

3%

exogenous

30.39

30.48

30.57

30.48

0.09

2.81

6.29

  

endogenous

25.92

25.79

25.84

25.85

0.07

  
 

1%

exogenous

31.49

31.41

32.13

31.68

0.40

1.12

11.55

  

endogenous

25.79

25.63

25.89

25.77

0.13

  

MON810

5%

exogenous

33.52

33.78

33.13

33.48

0.33

5.29

5.89

  

endogenous

25.84

25.89

25.95

25.89

0.06

  
 

3%

exogenous

34.67

34.13

34.25

34.35

0.28

3.20

6.76

  

endogenous

25.94

26.03

26.13

26.03

0.10

  
 

1%

exogenous

35.91

35.78

35.69

35.79

0.11

1.14

13.67

  

endogenous

25.84

25.89

26.19

25.97

0.19

  

Bt11

5%

exogenous

32.52

32.59

32.83

32.65

0.16

5.85

16.99

  

endogenous

25.99

26.08

26.13

26.07

0.07

  
 

3%

exogenous

33.41

33.64

33.29

33.45

0.18

3.18

5.74

  

endogenous

25.93

26.21

26.09

26.08

0.14

  
 

1%

exogenous

34.98

34.79

35.23

35.00

0.22

0.87

13.36

  

endogenous

25.94

26.05

25.83

34.22

0.18

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