Target gene/event | Sample | Endogenous/exogenous | Ct | Mean copies | SD | GM content (%) | Bias | ||
---|---|---|---|---|---|---|---|---|---|
 |  |  | Ct1 | Ct2 | Ct3 |  |  |  |  |
Screen specific detection | |||||||||
CaMV35s | 5% | exogenous | 34.42 | 34.17 | 34.08 | 34.22 | 0.18 | 4.33 | 13.37 |
 |  | endogenous | 26.9 | 26.89 | 26.78 | 26.86 | 0.07 |  |  |
 | 3% | exogenous | 35.01 | 34.84 | 34.67 | 34.84 | 0.17 | 2.81 | 6.22 |
 |  | endogenous | 26.78 | 26.82 | 26.93 | 26.84 | 0.08 |  |  |
 | 1% | exogenous | 36.56 | 36.21 | 36.89 | 36.55 | 0.34 | 0.86 | 13.87 |
 |  | endogenous | 26.81 | 26.79 | 26.88 | 26.83 | 0.05 |  |  |
NOS | 5% | exogenous | 32.46 | 32.38 | 32.1 | 32.31 | 0.19 | 4.80 | 3.92 |
 |  | endogenous | 26.91 | 26.73 | 26.83 | 26.82 | 0.09 |  |  |
 | 3% | exogenous | 33.21 | 33.32 | 33.07 | 33.2 | 0.13 | 2.67 | 10.92 |
 |  | endogenous | 26.81 | 26.92 | 26.97 | 26.9 | 0.08 |  |  |
 | 1% | exogenous | 34.37 | 34.29 | 34.78 | 34.48 | 0.26 | 1.15 | 15.12 |
 |  | endogenous | 26.93 | 26.99 | 27.13 | 27.02 | 0.10 |  |  |
Gene-specific detection | |||||||||
Cp4EPSPS | 5% | exogenous | 31.1 | 31.32 | 31.24 | 31.22 | 0.11 | 4.78 | 4.34 |
 |  | endogenous | 26.91 | 26.97 | 27.06 | 26.98 | 0.08 |  |  |
 | 3% | exogenous | 31.89 | 31.93 | 32.14 | 31.99 | 0.13 | 2.63 | 12.35 |
 |  | endogenous | 26.81 | 26.93 | 26.95 | 26.90 | 0.08 |  |  |
 | 1% | exogenous | 33.21 | 33.47 | 33.11 | 33.26 | 0.19 | 1.18 | 17.86 |
 |  | endogenous | 26.93 | 26.99 | 27.14 | 27.02 | 0.11 |  |  |
Cry1A(b) | 5% | exogenous | 29.32 | 29.13 | 28.92 | 29.123 | 0.20 | 4.04 | 19.22 |
 |  | endogenous | 25.78 | 25.81 | 25.73 | 25.7 | 0.04 |  |  |
 | 3% | exogenous | 29.42 | 29.28 | 29.35 | 29.35 | 0.07 | 3.50 | 16.70 |
 |  | endogenous | 25.69 | 25.81 | 25.92 | 25.81 | 0.12 |  |  |
 | 1% | exogenous | 31.45 | 31.39 | 31.26 | 31.37 | 0.10 | 0.83 | 16.97 |
 |  | endogenous | 25.83 | 25.86 | 25.91 | 25.87 | 0.04 |  |  |
Event-specific detection | |||||||||
MON863 | 5% | exogenous | 29.46 | 29.78 | 29.39 | 29.54 | 0.21 | 5.56 | 11.21 |
 |  | endogenous | 25.84 | 25.89 | 25.93 | 25.89 | 0.05 |  |  |
 | 3% | exogenous | 30.68 | 30.37 | 30.41 | 30.49 | 0.17 | 3.11 | 3.53 |
 |  | endogenous | 25.93 | 25.97 | 25.99 | 25.96 | 0.03 |  |  |
 | 1% | exogenous | 32.13 | 32.25 | 32.51 | 32.27 | 0.19 | 1.21 | 21.13 |
 |  | endogenous | 26.01 | 26.13 | 26.94 | 26.36 | 0.51 |  |  |
GA21 | 5% | exogenous | 29.81 | 29.78 | 29.53 | 29.71 | 0.15 | 5.62 | 12.37 |
 |  | endogenous | 26.07 | 26.09 | 25.95 | 26.04 | 0.08 |  |  |
 | 3% | exogenous | 30.39 | 30.48 | 30.57 | 30.48 | 0.09 | 2.81 | 6.29 |
 |  | endogenous | 25.92 | 25.79 | 25.84 | 25.85 | 0.07 |  |  |
 | 1% | exogenous | 31.49 | 31.41 | 32.13 | 31.68 | 0.40 | 1.12 | 11.55 |
 |  | endogenous | 25.79 | 25.63 | 25.89 | 25.77 | 0.13 |  |  |
MON810 | 5% | exogenous | 33.52 | 33.78 | 33.13 | 33.48 | 0.33 | 5.29 | 5.89 |
 |  | endogenous | 25.84 | 25.89 | 25.95 | 25.89 | 0.06 |  |  |
 | 3% | exogenous | 34.67 | 34.13 | 34.25 | 34.35 | 0.28 | 3.20 | 6.76 |
 |  | endogenous | 25.94 | 26.03 | 26.13 | 26.03 | 0.10 |  |  |
 | 1% | exogenous | 35.91 | 35.78 | 35.69 | 35.79 | 0.11 | 1.14 | 13.67 |
 |  | endogenous | 25.84 | 25.89 | 26.19 | 25.97 | 0.19 |  |  |
Bt11 | 5% | exogenous | 32.52 | 32.59 | 32.83 | 32.65 | 0.16 | 5.85 | 16.99 |
 |  | endogenous | 25.99 | 26.08 | 26.13 | 26.07 | 0.07 |  |  |
 | 3% | exogenous | 33.41 | 33.64 | 33.29 | 33.45 | 0.18 | 3.18 | 5.74 |
 |  | endogenous | 25.93 | 26.21 | 26.09 | 26.08 | 0.14 |  |  |
 | 1% | exogenous | 34.98 | 34.79 | 35.23 | 35.00 | 0.22 | 0.87 | 13.36 |
 |  | endogenous | 25.94 | 26.05 | 25.83 | 34.22 | 0.18 |  |  |