BMC Molecular Biology

Table 3 Repeatability and Reproducibility of CaMV35s and Nos AUDP PCR assays

From: A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids

True copy number	Ct values			Mean	SD^r	SD^R
	1	2	3
CaMV35s
100000	30.43	30.24	30.09	30.25	0.17	0.08
10000	33.19	33.25	33.38	33.27	0.09	0.04
1000	36.59	36.75	36.48	36.61	0.14	0.10
100	40.05	40.16	39.97	40.06	0.10	0.13
10	43.11	42.99	43.16	43.07	0.09	0.09
Nos
100000	30.22	30.12	30.28	30.21	0.08	0.05
10000	33.65	33.42	33.49	33.52	0.12	0.06
1000	36.94	36.58	36.84	36.79	0.19	0.11
100	40.66	40.98	40.75	40.80	0.17	0.09
10	43.19	43.56	43.31	43.35	0.19	0.14

SD^r, repeatability standard deviation; SD^R, reproducibility standard deviation.

Back to article page

ISSN: 1471-2199

Contact us

General enquiries: ORSupport@springernature.com