A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids

Table 2 Primers and probes of real-time PCR assays using TaqMan, UT and AUDP based on the genomic DNA sequence of pBI121 (GenBank No. AF485783).

Fluorescent real-time PCR	Orientation	Sequence^a (5'---3')	Amplicon length (bp)
TaqMan	Sense	CCGCCTTTGAGTGAGCTGATA	210
	Anti-sense	TGTAGACAACATCCCCTCCCC
	Probe	FAM CGCCGCAGCCGAACGACCGAG TAMRA
	Sense	GTATTACCGCCTTTGAGTGAGCT	510
	Anti-sense	ATGACAATCAGCTACTTCACTGTTG
	Probe	FAM CGCCGCAGCCGAACGACCGAG TAMRA
	Sense	CCGCCTTTGAGTGAGCTGATA	992
	Anti-sense	CTCCGCTGGTCCGATTGAAC
	Probe	FAM CGCCGCAGCCGAACGACCGAG TAMRA
	Sense	CCGCCTTTGAGTGAGCTGATA	1596
	Anti-sense	AATGGTTTCTGACGTATGTGCTTAG
	Probe	FAM CGCCGCAGCCGAACGACCGAG TAMRA
UT^a	Sense	taggaacaggcggcgacgaATACAA CATCCGCTTGCCCTCATC	195
	Anti-sense	TGCCAAAGGGTTCGTGTAG
	Sense	taggaacaggcggcgacga ATACAA CATCCGCTTGCCCTCATC	546
	Anti-sense	GGGATAACGCAGGAAAGAACAT
	Sense	CAGACGTGAAACCCAACATACC	1032
	Anti-sense	Taggaacaggcggcgacga ATACAA CCCAATAGCAGCCAGTCCCT
	Sense	taggaacaggcggcgacga ATACAA TCTGCTGTAGTGAGTGGGTTGC	1630
	Anti-sense	GCCGATTGTCTGTTGTGCC
	UT probe	FAM TCGTCGCCGCCTGTTCCTA TAMRA
AUDP probe^b	Sense	taggaacaggcggcgacgagcgtgggacataAA ACTCCATCCGCTTGCCCTCATC	195
	Anti-sense	TGCCAAAGGGTTCGTGTAG
	Sense	taggaacaggcggcgacgagcgtgggacataAAACTC CATCCGCTTGCCCTCATC	546
	Anti-sense	GGGATAACGCAGGAAAGAACAT
	Sense	CAGACGTGAAACCCAACATACC	1032
	Anti-sense	taggaacaggcggcgacgagcgtgggacataAAACTC CCCAATAGCAGCCAGTCCCT
	Sense	taggaacaggcggcgacgagcgtgggacataAAACCTC TCTGCTGTAGTGAGTGGGTTGC	1630
	Anti-sense	GCCGATTGTCTGTTGTGCC
	FP	FAM TAGGAACAGGCGGCGACGAGCGTGGGACATA
	QP	AGTCGTCGCCGCCTGTTCCTA Dabcyl

^aUT sequence: taggaacaggcggcgacga, italicized nucleotide sequences are the linker region of the UT PCR primer between the UT sequence and target specific DNA primer.
^bUT sequence: taggaacaggcggcgacgagcgtgggacata, italicized nucleotide sequences are the linker region of UT sequence and target specific DNA primer.

ISSN: 1471-2199