A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids

Yang, Litao; Liang, Wanqi; Jiang, Lingxi; Li, Wenquan; Cao, Wei; Wilson, Zoe A; Zhang, Dabing

doi:10.1186/1471-2199-9-54

Table 1 Primer pairs and universal duplex probes used in duplex PCR in this study^a

From: A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids

Application	Target Gene	Orientation	Sequence (5'---3')	Amplicon Length (bp)
GMO quantification	CaMV35s promoter	Sense	taggaacaggcggcgacgagcgtgggacataATACAA GACGCACAATCCCACTATC	68
		Anti-sense	CCTCTCCAAATGAAATGAACT
	NOS terminator	Sense	taggaacaggcggcgacgagcgtgggacataATACAA ATGTATAATTGCGGGACTCTA	52
		Anti-sense	TGACGTTATTTATGAGATGGGT
	cp4EPSPS	Sense	taggaacaggcggcgacgagcgtgggacata ATACAA GGAACGTCTTCTTTTTCCACG	199
		Anti-sense	CTTATTGCATTTCATTCAAAATAAG
	Cry1A(b)	Sense	taggaacaggcggcgacgagcgtgggacataATACAA CCCCCCTCAGAACAACAA	56
		Anti-sense	GGCTCAGACGGTGGCT
	GA21	Sense	CTTATCGTTATGCTATTTGCAACTTTAGA	112
		Anti-sense	atccttgtccgccgctgctcgcaccctgtatATA TGGCTCGCGATCCTCCT
	Bt11	Sense	taggaacaggcggcgacgagcgtgggacataATA GCGGAACCCCTATTTGTTTATT	112
		Anti-sense	atccttgtccgccgctgctcgcaccctgtatCGC ACACCTACAGATTTTAGACCAA
	MON863	Sense	taggaacaggcggcgacgagcgtgggacata ATA GGTTGGTTGGTGAGCCTAGTGA	130
		Anti-sense	CATCCGAACAAGTAGGGTCAAT
	MON810	Sense	taggaacaggcggcgacgagcgtgggacata ATA TCTTTCAAGCCGAAGGTACATC	177
		Anti-sense	GCGAAGGTTATGAAGGACATAC
Gene expression analysis	Actin	Sense	TGAAGTTCTGTTCCAGCCATCC	171
		Anti-sense	atccttgtccgccgctgctcgcaccctgtatCGC TTACTCATCCTATCACCAATCCC
	SPL	Sense	TCCTCCGATGAACGGCTAC	70
		Anti-sense	atccttgtccgccgctgctcgcaccctgtatGGAAACCTTGGCTCCTCTG
	EMS1	Sense	taggaacaggcggcgacgagcgtgggacataTTT CCTACATCACGGTTTCATCC	197
		Anti-sense	CTCGGGCACTCTGACCATAC
	AMS	Sense	taggaacaggcggcgacgagcgtgggacataAAA TTGCGAATACAAACCAGGAG	102
		Anti-sense	ACCAGGCTGAGGTAGCGAGT
	TPD1	Sense	CGTCTCCGTTGAAGCCTCC	188
		Anti-sense	atccttgtccgccgctgctcgcaccctgtatAAA CACCACTATGTCCGTACTCT
	DYT1	Sense	CCAGTGAGGATGAGCCGTAG	133
		Anti-sense	atccttgtccgccgctgctcgcaccctgtatAAT GAGATCGCAGAGCCATAAGC
Genotype identification	ms1 mutant	Sense	taggaacaggcggcgacgagcgtgggacataTTA CCGGTTTAAAGGCCAGa	251
		Anti-sense	ATGGCTCTCCTTTTGCTAC
	AUDP	FP	FAM TAGGAACAGGCGGCGACGAGCGTGGGACATA
		QP	AGTCGTCGCCGCCTGTTCCTA Dabcyl

^aUT sequences: taggaacaggcggcgacgagcgtgggacata, italicized nucleotide sequences are the linker region of the modified target specific primer between the UT sequence and target specific DNA primer.

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ISSN: 1471-2199

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