Figure 4

Duplex fluorogenic PCR analysis for RRS detection employing the endogenous reference gene Lectin and exogenous gene cp4EPSPS. (A) Amplification plot of endogenous Lectin gene. Each dilution contained 100 ng of total soybean DNA. (B) Amplification plot of the transgenic cp4EPSPS gene, five different GM contents (10.0%, 5.0%, 1.0%, 0.5% and 0.1%) of soybean DNA that contained 10, 5, 1, 0.5 and 0.1 ng GM soybean DNA per reaction, respectively, were plotted as the initial RRS DNA concentration versus Ct standard curve (R² = 0.9964). (C) Standard curve, plotting log [GMOs concentration] versus change in threshold of detection (Δ Ct), R² = 0.9964.