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Figure 1 | BMC Molecular Biology

Figure 1

From: A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids

Figure 1

Schematic representation of the mechanism of AUDP PCR assay. (A) The structure of the duplex probes and modified target specific primers; (B) The first cycle of PCR amplification; (C) The second cycle of amplification. (D) The third cycle of amplification and fluorescent signal generation; (E), (Fa) and (Fb), The fourth cycle of amplification and fluorescent signal generation.

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