Figure 1From: A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acidsSchematic representation of the mechanism of AUDP PCR assay. (A) The structure of the duplex probes and modified target specific primers; (B) The first cycle of PCR amplification; (C) The second cycle of amplification. (D) The third cycle of amplification and fluorescent signal generation; (E), (Fa) and (Fb), The fourth cycle of amplification and fluorescent signal generation.Back to article page