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Table 1 Primer sequences and amplicon characteristics

From: Selection of reference genes for quantitative real-time PCR in a rat asphyxial cardiac arrest model

Gene Sequence Reference a Position cDNA b Position gene Amplicon length (bp) Product TMe, f (°C) PCR efficiencyf (%)
Gapdh 5'-CAACTCCCTCAAGATTGTCAGCAA-3' NM_017008 493–516 Int. span. 118 82.9 105
  5-'GGCATGGACTGTGGTCATGA-3' NC_005103 610-591     
Actb c 5'-AAGTCCCTCACCCTCCCAAAAG-3' V01217 3474–3495 Exon 97 82.9 92.5
  5'-AAGCAATGCTGTCACCTTCCC-3' V01217 3570-3550     
CypA c 5'-TATCTGCACTGCCAAGACTGAGTG-3' M19533 381–404 Int. span. 126 82.0 98
  5'-CTTCTTGCTGGTCTTGCCATTCC-3' NW_047430 507-485     
B2m 5'-CGAGACCGATGTATATGCTTGC-3' NM_012512 286–307 Int. span. 114 79.8 92
  5'-GTCCAGATGATTCAGAGCTCCA-3' NC_005102 399-378     
Rpl13a 5'-GGATCCCTCCACCCTATGACA-3' NM_173340 334–354 Int. span. 132 83.5 100
  5'-CTGGTACTTCCACCCGACCTC-3' NC_005100 464-444     
Hprt c 5'-CTCATGGACTGATTATGGACAGGAC-3' NM_012583 179–203 Int. span. 123 80.9 97
  5'-GCAGGTCAGCAAAGAACTTATAGCC-3' NW_048050 301-277     
Ywhaz 5'-GATGAAGCCATTGCTGAACTTG-3' NM_013011 955–976 Int. span. 117 77.6 95
  5'-GTCTCCTTGGGTATCCGATGTC-3' NC_005106 1071-1050     
Sdha 5'-TCCTTCCCACTGTGCATTACAA-3' NM_130428 1222–1243 Int. span. 105 81.4 98
  5'-CGTACAGACCAGGCACAATCTG-3' NC_005100 1326-1305     
Pgk1 5'-ATGCAAAGACTGGCCAAGCTAC-3' NM_053291 969–990 Int. span. 104 81.9 99
  5'-AGCCACAGCCTCAGCATATTTC-3' NC_005120 1072-1051     
Tbp 5'-TGGGATTGTACCACAGCTCCA-3' NM_001004198 679–699 Int. span. 131 78.2 100
  5'-CTCATGATGACTGCAGCAAACC-3' NC_005100 810-789     
Mac-1 d 5'-CTGCCTCAGGGATCCGTAAAG-3' NM_012711 680–700 Int. span. 150 79.8 101
  5'-CCTCTGCCTCAGGAATGACATC-3' NW_047562 829-808     
  1. a Genebank accession number of cDNA (upper line) and genomic sequences or contigs (lower line), b Position of amplification product within cDNA sequence (genomic sequence for Actb), Exon; both primers bind to the same exon, Int. span.; primers bind on different exons, c Primer sequences according to [46], d Primer sequences according to [47], e melting temperature of specific PCR product, f calculated by MxPro Mx3005P v3.00 software (Stratagene, La Jolla, CA)