Table 1 Primer sequences and amplicon characteristics
From: Selection of reference genes for quantitative real-time PCR in a rat asphyxial cardiac arrest model
| Gene | Sequence | Reference a | Position cDNA b | Position gene | Amplicon length (bp) | Product TMe, f (°C) | PCR efficiencyf (%) |
|---|---|---|---|---|---|---|---|
| Gapdh | 5'-CAACTCCCTCAAGATTGTCAGCAA-3' | NM_017008 | 493–516 | Int. span. | 118 | 82.9 | 105 |
| 5-'GGCATGGACTGTGGTCATGA-3' | NC_005103 | 610-591 | |||||
| Actb c | 5'-AAGTCCCTCACCCTCCCAAAAG-3' | V01217 | 3474–3495 | Exon | 97 | 82.9 | 92.5 |
| 5'-AAGCAATGCTGTCACCTTCCC-3' | V01217 | 3570-3550 | |||||
| CypA c | 5'-TATCTGCACTGCCAAGACTGAGTG-3' | M19533 | 381–404 | Int. span. | 126 | 82.0 | 98 |
| 5'-CTTCTTGCTGGTCTTGCCATTCC-3' | NW_047430 | 507-485 | |||||
| B2m | 5'-CGAGACCGATGTATATGCTTGC-3' | NM_012512 | 286–307 | Int. span. | 114 | 79.8 | 92 |
| 5'-GTCCAGATGATTCAGAGCTCCA-3' | NC_005102 | 399-378 | |||||
| Rpl13a | 5'-GGATCCCTCCACCCTATGACA-3' | NM_173340 | 334–354 | Int. span. | 132 | 83.5 | 100 |
| 5'-CTGGTACTTCCACCCGACCTC-3' | NC_005100 | 464-444 | |||||
| Hprt c | 5'-CTCATGGACTGATTATGGACAGGAC-3' | NM_012583 | 179–203 | Int. span. | 123 | 80.9 | 97 |
| 5'-GCAGGTCAGCAAAGAACTTATAGCC-3' | NW_048050 | 301-277 | |||||
| Ywhaz | 5'-GATGAAGCCATTGCTGAACTTG-3' | NM_013011 | 955–976 | Int. span. | 117 | 77.6 | 95 |
| 5'-GTCTCCTTGGGTATCCGATGTC-3' | NC_005106 | 1071-1050 | |||||
| Sdha | 5'-TCCTTCCCACTGTGCATTACAA-3' | NM_130428 | 1222–1243 | Int. span. | 105 | 81.4 | 98 |
| 5'-CGTACAGACCAGGCACAATCTG-3' | NC_005100 | 1326-1305 | |||||
| Pgk1 | 5'-ATGCAAAGACTGGCCAAGCTAC-3' | NM_053291 | 969–990 | Int. span. | 104 | 81.9 | 99 |
| 5'-AGCCACAGCCTCAGCATATTTC-3' | NC_005120 | 1072-1051 | |||||
| Tbp | 5'-TGGGATTGTACCACAGCTCCA-3' | NM_001004198 | 679–699 | Int. span. | 131 | 78.2 | 100 |
| 5'-CTCATGATGACTGCAGCAAACC-3' | NC_005100 | 810-789 | |||||
| Mac-1 d | 5'-CTGCCTCAGGGATCCGTAAAG-3' | NM_012711 | 680–700 | Int. span. | 150 | 79.8 | 101 |
| 5'-CCTCTGCCTCAGGAATGACATC-3' | NW_047562 | 829-808 |