Figure 6From: IRES-mediated translation of the carboxy-terminal domain of the horizontal cell specific connexin Cx55.5 in vivo and in vitroConfirmation of the activity of the IRES element by western blot analysis. A) Schematic representation of the Di-cis vectors with EGFP as second cistron. (I) indicates pRE as control vector, (II) pREΔCMV with the corresponding promoterless control vector, (III) shows the pRE-IR2 with IRES element IR2 (nt631 to nt990) in the inter-cistronic region, and (IV) the corresponding promoterless construct (pRE-IR2ΔCMV). B) Western blot of the expression product of the Di-cis constructs transiently transfected in N2A cells: The blots show the increased expression of the downstream EGFP cistron mediated by the IR2 element (III). C) Western blot of β-actin as loading control.Back to article page