Northern blot (A) and RT-PCR (B) analyses of the SeTre-2 transcript in different tissues of fifth instar larvae of S. exigua. (A) For Northern blot analysis, total RNA was extracted from various tissues: brain (1), fat body (2), cuticle (3), Malpighian tubules (4), and midgut (5). Probes specific for SeTre-2 were radiolabeled using [α-32P]dCTP. (B) RT-PCR analysis of DL2000 marker (M), midgut (1), brain (2), Malpighian tubules (3), cuticle (4), fat body (5), trachea (6), and spermary (7). β-Actin was used a loading control and visualization was by ethidium bromide staining.