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Table 1 Oligonucleotide Sequences for PCR and EMSA*

From: Comparative in silico analysis identifies bona fide MyoD binding sites within the Myocyte Stress 1 gene promoter

Cloning PCR
P-1585-SacI 5'-TATTCAATGCTTAGTCCTGC-3'
P+60-HindIII 5'-CCAAGCTTCAGGCTACCTGTTTCTTCTC-3'
Site Directed Mutagenesis PCR
ΔT ATA Fw 5'-CACCCTTTCACACCCTGCTTCTG TTTAAATCCCAGGCAACTC-3'
ΔT ATA Rv 5 '-GAGTTGCCTGGGATTTAAAC AGAAGCAGGGTGTGAAAGGGTG-3'
ΔE1 Fw 5 '-CACTGAACAGGTGCTGTTTCTCTGTCGTTAAG ACTTATCCTTTCAG TTCTCTTAAAA-3'
ΔE1 Rv 5 '-TTTTAAGAGAACTGAAAGGATAAGTCTTAAC GACAGAGAAACAGCA CCTGTTCAGTG-3'
ΔE2 Fw 5'-CTTTCCACCCTGGCG CGG GGAGAAGAAAGGAG-3'
ΔE2 Rv 5'-CTCCTTTCTTCTCCCC GC GCCAGGGTGGAAAG-3'
ΔE3 Fw 5 '-CAAGGAAAACATAAAGCTAAGCG CGG GATTCAATCTAGTACTTC-3'
ΔE3 Rv 5 '-GAAGTACTAGATTGAATCCC GC GCTTAGCTTTATGTTTTCCTTG-3'
Quantitative PCR
MS1 Fw 5'-GTGACAGCATAGACACAGAGGAC-3'
MS1 Rv 5'-CACTGCTGCCCACCTGCCTT-3'
EF1-α Fw 5'-AGCTTCTCTGACTACCCTCCACTT-3'
EF1-α Rv 5'-GACCGTTCTTCCACCACTGATT-3'
ChIP Quantitative PCR
E1-1526 5'-CACATTTTTATCTGGTCTAATACACTG-3'
E1-1482 5'-ATTTTTAATAGAACTGAAAAGAGAAGTCA-3'
E2-281 5'-TAAGGTCAAGGAAAACATAAAGCTA-3'
E2-190 5'-ACGGATATGTTCCCTCCTCTCTC-3'
EMSA
E-Consensus 5'-CCCTTGGAACATCTGTCGATGCTG-3'
E1-MS1 5'-TTCTCTGTCCACATGACTTATCCT-3'
E2-MS1 5'-ACCCTGGCACTTGGAGAAGAA-3'
E3-MS1 5'-AGCTAAGCACATGATTCAATC-3'
  1. *Mutated oligonucleotides are indicated in bold and underlined.