Table 1 Oligonucleotide Sequences for PCR and EMSA*
Cloning PCR | |
|---|---|
P-1585-SacI | 5'-TATTCAATGCTTAGTCCTGC-3' |
P+60-HindIII | 5'-CCAAGCTTCAGGCTACCTGTTTCTTCTC-3' |
Site Directed Mutagenesis PCR | |
ΔT ATA Fw | 5'-CACCCTTTCACACCCTGCTTCTG TTTAAATCCCAGGCAACTC-3' |
ΔT ATA Rv | 5 '-GAGTTGCCTGGGATTTAAAC AGAAGCAGGGTGTGAAAGGGTG-3' |
ΔE1 Fw | 5 '-CACTGAACAGGTGCTGTTTCTCTGTCGTTAAG ACTTATCCTTTCAG TTCTCTTAAAA-3' |
ΔE1 Rv | 5 '-TTTTAAGAGAACTGAAAGGATAAGTCTTAAC GACAGAGAAACAGCA CCTGTTCAGTG-3' |
ΔE2 Fw | 5'-CTTTCCACCCTGGCG CGG GGAGAAGAAAGGAG-3' |
ΔE2 Rv | 5'-CTCCTTTCTTCTCCCC GC GCCAGGGTGGAAAG-3' |
ΔE3 Fw | 5 '-CAAGGAAAACATAAAGCTAAGCG CGG GATTCAATCTAGTACTTC-3' |
ΔE3 Rv | 5 '-GAAGTACTAGATTGAATCCC GC GCTTAGCTTTATGTTTTCCTTG-3' |
Quantitative PCR | |
MS1 Fw | 5'-GTGACAGCATAGACACAGAGGAC-3' |
MS1 Rv | 5'-CACTGCTGCCCACCTGCCTT-3' |
EF1-α Fw | 5'-AGCTTCTCTGACTACCCTCCACTT-3' |
EF1-α Rv | 5'-GACCGTTCTTCCACCACTGATT-3' |
ChIP Quantitative PCR | |
E1-1526 | 5'-CACATTTTTATCTGGTCTAATACACTG-3' |
E1-1482 | 5'-ATTTTTAATAGAACTGAAAAGAGAAGTCA-3' |
E2-281 | 5'-TAAGGTCAAGGAAAACATAAAGCTA-3' |
E2-190 | 5'-ACGGATATGTTCCCTCCTCTCTC-3' |
EMSA | |
E-Consensus | 5'-CCCTTGGAACATCTGTCGATGCTG-3' |
E1-MS1 | 5'-TTCTCTGTCCACATGACTTATCCT-3' |
E2-MS1 | 5'-ACCCTGGCACTTGGAGAAGAA-3' |
E3-MS1 | 5'-AGCTAAGCACATGATTCAATC-3' |