Figure 8From: Regulation of mouse Scgb3a1 gene expression by NF-Y and association of CpG methylation with its tissue-specific expressionEMSA with methylated CpG oligunucleotides. EMSA was carried out using nuclear extracts prepared from mtCC or Hepa 1 cells and oligonucleotides containing proximal or distal CpG site methylated (Me +) or unmethylated (Me -). Oligonucleotide sequences are shown at the bottom with methylated C underlined in boldface. Shifted bands only seen with methylated CpG oligonucleotide are shown by open arrowhead for proximal or asterisk for distal CpG region.Back to article page