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Figure 5 | BMC Molecular Biology

Figure 5

From: Regulation of mouse Scgb3a1 gene expression by NF-Y and association of CpG methylation with its tissue-specific expression

Figure 5

NF-Y controls expression of Scgb3a1 gene. (A) ChIP analysis of mtCC, NIH3T3, and MLE15 cells for endogenous NF-Y binding. Antibodies used were NF-YA and IgG as a control. A sequence derived from the exon II of Scgb3a2, a homologous gene to Scgb3a1 was used as a reference gene. Illustration of the PCR strategy is shown at the bottom. Experiments were repeated twice and the same results were obtained. (B) Western blot analysis of NF-YA protein level after NF-YA shRNA transfection into mtCC cells. Experiments were repeated twice and the same results were obtained. Representative western blot result is shown with the quantitated bar graph at lower panel. (C) Northern blot analysis of SCGB3A1 mRNA level after NF-YA shRNA transfection into mtCC cells. Experiments were repeated twice and the same results were obtained. Representative northern blot result is shown with the quantitated bar graph at lower panel.

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