Figure 2

NF-Y comprised of all three subunits is required for mouse Scgb3a1 gene transcription. (A) Transient transfection of serial deletion luciferase reporter constructs into COS-1 cells in the absence (control), or presence of NF-Y or its dominant negative form NFY(Am29). Relative luciferase activity is shown as the mean ± SD based on the activity of pGL4 (shown as construct 0) as 1 from three independent experiments, each carried out in duplicate. (B) -598 reporter construct was co-transfected with various combination of NF-Y subunits into COS-1 cells. Relative luciferase activity is shown as the mean ± SD based on the activity of construct -598 without any NF-Y subunits as 1 from three independent experiments, each carried out in duplicate.