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Table 5 Statistical output from BestKeeper analysis.

From: Exercise induced stress in horses: Selection of the most stable reference genes for quantitative RT-PCR normalization

  ACTB B2M GAPDH HPRT R18S RPL32 SDHA TFRC UBB
N 30 30 30 30 30 30 30 30 30
G Mean [Ct] 17.85 19.84 23.20 25.34 9.07 21.15 24.51 26.54 19.25
A Mean [Ct] 17.86 19.85 23.22 25.35 9.10 21.15 24.52 26.56 19.26
Min [Ct] 16.92 18.55 20.99 24.11 7.97 20.10 23.22 25.11 17.94
Max [Ct] 19.23 21.86 25.05 27.76 12.02 22.42 26.91 28.88 21.46
SD [± Ct] 0.49 0.51 0.71 0.62 0.57 0.46 0.66 0.73 0.64
CV [% Ct] 2.77 2.57 3.08 2.43 6.28 2.18 2.70 2.75 3.35
coeff. of corr. [r] 0.890 0.921 0.708 0.963* 0.949* 0.832 0.983* 0.844 0.935*
p-value 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001
  1. Genes are listed in alphabetical order. N: number of samples; G Mean [Ct]: geometric mean of the Ct; A Mean [Ct]: arithmetic mean of the Ct; Min and Max [Ct]: extreme values of the Ct; SD [Ct]: standard deviation of the Ct; CV [% Ct]: coefficient of variance expressed as percentage on the Ct level. * Indicates the best correlation between control genes and the BestKeeper index.