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Table 3 Candidate reference genes ranking according to geNorm.

From: Exercise induced stress in horses: Selection of the most stable reference genes for quantitative RT-PCR normalization

Rank

Gene Symbol

M value

V value

1/2

SDHA/HPRT

0.232

---

3

R18S

0.274

0.090

4

B2M

0.311

0.078

5

UBB

0.335

0.064

6

ACTB

0.376

0.068

7

RPL32

0.405

0.059

8

TFRC

0.448

0.065

9

GAPDH

0.521

0.080

  1. M value is calculated as average expression stability of control genes during stepwise exclusion of the least stable controls. V value is calculated as the pair-wise variation between two sequential normalization.
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BMC Molecular Biology

ISSN: 1471-2199

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